Connectedness to nature involves the emotional relationship of the individual and the natural environment, based on the theory of biophilia, the innate tendency of the individual to connect with living beings and systems. Biophilia (love of nature) and biophobia (fear of nature) are significant factors in the development of environmental sensitivity, education for the environment and sustainable development. Education for the environment and sustainable development are listed in the National Curriculum for Early and Preschool Education as fundamental educational values. In this way, the examination of biophilia and biophobia becomes important for educators and teachers to be able to apply this knowledge in practice in order to develop a society that enjoys the benefits of the natural environment, but also to develop a sense of responsibility for their preservation. The aim of this research was to evaluate the emotional attributions that preschool children attach to nature and its elements, to investigate biophilia and biophobia of preschool children. The research was conducted with 105 (N = 105) children from five mixed kindergarten groups attending the kindergarten in Međimurje and Kindergarten Drenova. For the purpose of conducting the research, a measuring instrument was designed based on the research of Olivos-Jar et al. (2020) which studied the connectedness of preschool children to nature, ie the emotional relationship between the individual and the natural environment, based on biophilia theory. The tasks of the research were to determine the self-assessment of emotions of preschool children in relation to outdoor natural spaces, insects, domestic animals, wild animals and nature pollution. The results of this research showed that children mainly express emotions of happiness according to photographs of domestic animals and outdoor natural spaces, and to photographs of insects and wild animals negative emotions are expressed for specific animals (eg spider, bee, snake, shark) similar researches has shown that they cause fear of stings or bites. Furthermore, negative emotions (fear, sadness and anger) were also evoked by photographs of nature pollution indicating the biophilia of preschool children.