The period of childhood is complex in many aspects especially for a wide range of knowledge that is adopted and improved in that era of life. Biotic motor skills are a prerequisite for the optimal growth and development of each individual, and the adoption of motor skills and abilities affects the quality of life in adulthood. The main purpose of this research is to determine the levels of learning biotic motor skills for overcoming obstacles in early and pre-school children, while identifying significant differences considering the age and gender of the respondents, and considering their inclusion in the educational program enriched with sports facilities. The study was conducted on a sample of 110 early and pre-school subjects, of which 59 were boys and 51 were girls. Of the total number of respondents, 79 were included in sports programs. For the purpose of this research, polygon of biotic motor knowledge for overcoming obstacles was developed. It was conducted among the children respondents with the remaining 10 tests to assess the anthropological status of the children. The research was conducted in six educational groups of the Kindergarten “Rijeka”; “Mavrica” and “Drenova” kindergarten. The results of the study indicate that there is no statistically significant difference between male and female respondents. Also, the statistical analysis eliminates a significant difference in regard to the subjects' involvement in the sports program, except for the biotic motor skills polygon test for overcoming obstacles in which children involved in the sports program show greater success and ease in solving motor tasks. A statistically significant difference is evident in the differences between the age groups of the respondents, which indicates the correlation between the respondents' performance and their age, where they are proportionally related. Higher performance and learning levels of biotic motor skills are present in older children. The research points to the importance of using kinesiological activities from a child's earliest age, which significantly contributes to the optimal growth and development of the individual and affects the quality of life.