This paper will explain the role of Education for Sustainable Development in pre-schools as well as terms related to the competences, Initial Education and professional development of pre- school teachers for Sustainable Development. The aim is to analyze the extent to which pre-school teachers possess competences in Education for Sustainable Development, in which forms of education/professional development have they built up their current level of competences and what is their willingness to acquire new competences through various forms of further professional development. The survey was conducted among pre-school teachers in Kindergarten „Radost“ in Crikvenica with its regional kindergartens, Kindergarten „Orepčići“ in Kraljevica, Kindergarten „Cvrčak i Mrav“ in Tribalj with its regional kindergarten in Bribir, as well as in Kindergarten „Fijolica“ in Novi Vinodolski. There search results have pointed out that most of the pre- school teachers possess competences in Education for Sustainable Development and that their competences are largely developed by self improvement. Also, the results showed the need for a teacher’s stronger involvement in the various forms of professional training for Sustainable Development.